HS-AFM Movies

A pentameric TRPV3 channel with a dilated pore
Nature, 2023, 621(7977), 206–214, doi:10.1038/s41586-023-06470-1
Shifra Lansky, Jingying Zhang, John Michael Betancourt, Yining Jiang, Elizabeth D Kim, Navid Paknejad, Crina M Nimigean, Peng Yuan, Simon Scheuring*

Supplementary Video 3
Supplementary Video 5
Supplementary Video 13
4mer-5mer-transition

Membrane-mediated protein interactions drive membrane protein organization
Nature Communications, 2022, 13(7373), doi.org/10.1038/s41467-022-35202-8
Yining Jiang, Batiste Thienpont, Vinay Sapuru, Richard K Hite, Jeremy Dittman, James N Sturgis, Simon Scheuring*

S4_AqpZ_array_dynamics_210911_YJ_AdobeExpress
S1_Aqpz_experiment_design_210911_YJ_AdobeExpress

Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism
Nature Communications, 2022, 13(5039). doi.org/10.1038/s41467-022-32757-4
Fang Jiao*, François Dehez, Tao Ni, Xiulian Yu, Jeremy Dittman, Robert Gilbert, Christophe Chipot, Simon Scheuring*

___PFN2_PrePore-To-Pore_Transition

Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change
ACS Nanoscience Au, 2022, doi.org/10.1021/acsnanoscienceau.2c00019
Mohit Sharma, Artur P. Biela, Agnieszka Kowalczyk, Kinga Borzęcka-Solarz, Bernard M. A. G. Piette, Szymon Gaweł, Joshua Bishop, Philipp Kukura, Justin L. P. Benesch, Motonori Imamura, Simon Scheuring & Jonathan G. Heddle*

ng2c00019_si_003
ng2c00019_si_002

Snf7 spirals sense and alter membrane curvature
Nature Communications, 2022, 13(2174):doi.org/10.1038/s41467-022-29850-z
Nebojsa Jukic#, Alma P. Perrino#, Aurélien Roux & Simon Scheuring*

Supplementary Movie 3
Supplementary Movie 1
Supplementary Movie 2
Supplementary Movie 6
Supplementary Movie 4
Supplementary Movie 5
Supplementary Movie 7
Supplementary Movie 9
Supplementary Movie 8
Supplementary Movie 11
Supplementary Movie 12
Supplementary Movie 14
Supplementary Movie 15
Supplementary Movie 16
Supplementary Movie 17
Supplementary Movie 13

Chemically induced protein cage assembly with programmable opening and cargo release
Science Advances, 2022, 8(1):eabj9424, doi.org/10.1126/sciadv.abj9424
Izabela Stupka, Yusuke Azuma, Artur P Biela, Motonori Imamura, Simon Scheuring, Elżbieta Pyza, Olga Woźnicka, Daniel P Maskell & Jonathan G Heddle*

sciadv.abj9424_movie_s3
sciadv.abj9424_movie_s1
sciadv.abj9424_movie_s2

Single molecule kinetics of bacteriorhodopsin by HS-AFM
Nature Communications, 2021, 12(7225), doi.org/10.1038/s41467-021-27580-2
Alma P. Perrino, Atsushi Miyagi & Simon Scheuring*

Perrino_NatComm_2021_03
Perrino_NatComm_2021_01
Perrino_NatComm_2021_02
Perrino_NatComm_2021_04
Perrino_NatComm_2021_05

Correlation of Membrane Protein Conformational and Functional Dynamics
Nature Communications, 2021, 12(4363), doi.org/10.1038/s41467-021-24660-1
Raghavendar Reddy Sanganna Gari, Joel José Montalvo‐Acosta, George R Heath, Xiaolong Gao, Crina M Nimigean, Christophe Chipot* & Simon Scheuring*

Sanganna_Gari_NatComm_2021_01

Localization Atomic Force Microscopy
Nature, 2021, 594(7863):385–390, doi:10.1038/s41586-021-03551-x
George Heath, Ekaterina Kots, Janice Robertson, Shifra Lansky, George Khelashvili, Harel Weinstein & Simon Scheuring*

Heath_Nature_2021_01
Heath_Nature_2021_02
Heath_Nature_2021_04
Heath_Nature_2021_03

The hierarchical assembly of septins revealed by high-speed AFM
Nature Communications, 2020, 11(5062), doi.org/10.1038/s41467-020-18778-x
Fang Jiao, Kevin S Cannon, Yi-Chih Lin, Amy S Gladfelter & Simon Scheuring*

Jiao_NatComm_2020_05
Jiao_NatComm_2020_03
Jiao_NatComm_2020_04
Jiao_NatComm_2020_06
Jiao_NatComm_2020_07
Jiao_NatComm_2020_01
Jiao_NatComm_2020_02

Millisecond dynamics of an unlabeled amino acid transporter
Nature Communications, 2020, 11(5016), doi.org/10.1038/s41467-020-18811-z
Tina R Matin#, George R Heath#, Gerard HM Huysmans, Olga Boudker  & Simon Scheuring*

Matin_NatComm_2020_01
Matin_NatComm_2020_02
Matin_NatComm_2020_03

Annexin-A5 Stabilizes Membrane Defects by Inducing Lipid Phase Transition
Nature Communications, 2020, 11(230), doi:10.1038/s41467-019-14045-w
Yi-Chih Lin, Christophe Chipot & Simon Scheuring*

A5_NatComm2020_Movie7
A5_NatComm2020_Movie4
A5_NatComm2020_Movie5
A5_NatComm2020_Movie2
A5_NatComm2020_Movie1
A5_NatComm2020_Movie3
A5_NatComm2020_Movie6

Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity
Science Advances, 2020, 6(5), doi: 10.1126/sciadv.aax8286
Tao Ni#, Fang Jiao#, Xiulian Yu#, Saša Aden, Lucy Ginger, Sophie Williams, Fangfang Bai, Vojta Prazak, Dimple Karia, Phillip Stansfeld, Peijun Zhang, George Munson, Gregor Anderluh, Simon Scheuring*, Robert JC Gilbert*

PFN2_SciAdv2020_Movie1
PFN2_SciAdv2020_Movie3
PFN2_SciAdv2020_Movie2
PFN2_SciAdv2020_Movie4

Real time dynamics of gating-related conformational changes in CorA
eLife, 2019, 8:e47322 doi: 10.7554/eLife.47322
Martina Rangl, Nicolaus Schmandt, Eduardo Perozo* & Simon Scheuring*

elife-47322-video3
elife-47322-video2
elife-47322-video1
elife-47322-video5
elife-47322-video7
elife-47322-video4
elife-47322-video6
elife-47322-video8

Force-induced conformational changes in Piezo1
Nature, 2019, 573(7773):230–234, doi. 10.1038/s41586-019-1499-2
Yi-Chih Lin#, Yusong R Guo#, Atsushi Miyagi, Jesper Levring, Roderick MacKinnon* & Simon Scheuring*

Piezo1_Nature2019_Movie1
Piezo1_Nature2019_Movie2
Piezo1_Nature2019_Movie3

High-Speed AFM Height Spectroscopy (HS-AFM-HS): Microsecond dynamics of unlabeled biomolecules
Nature Communications, 2018, 9(1):4983. doi:10.1038/s41467-018-07512-3
George Heath & Simon Scheuring*

2018-4 George Heath Nature Communications-1
2018-4 George Heath Nature Communications-2

An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel
Nature Communications, 2018, 9(1):3978. doi:10.1038/s41467-018-06414-8.
Arin Marchesi, Xiaolong Gao, Ricardo Adaixo, Jan Rheinberger, Henning Stahlberg, Crina Nimigean* & Simon Scheuring*

2018-3 Arin Marchesi Nature Communicatioins-3
2018-3 Arin Marchesi Nature Communicatioins-1
2018-3 Arin Marchesi Nature Communicatioins-2

Structural titration of receptor ion channel GLIC gating by HS-AFM
PNAS, 2018, doi.org/10.1073/pnas.1805621115
Yi Ruan, Kevin Kao, Solène Lefebvre, Arin Marchesi, Pierre-Jean Corringer Richard K Hite & Simon Scheuring*

2018-2 Yi Ruan PNAS-3s
2018-2 Yi Ruan PNAS-4s

High-speed AFM reveals the inner workings of the MinDE protein oscillator
Nano Letters, 2018, 18(1):288–296, doi:10.1021/acs.nanolett.7b04128
Atsushi Miyagi#, Beatrice Ramm#, Petra Schwille & Simon Scheuring*

2018-1 Atsushi Miyagi Nano Letters-4
2018-1 Atsushi Miyagi Nano Letters-2
2018-1 Atsushi Miyagi Nano Letters-5
2018-1 Atsushi Miyagi Nano Letters-3
2018-1 Atsushi Miyagi Nano Letters-1

Lysenin toxin membrane insertion is pH-dependent and non-cooperative
Biophys J, 2017, 113(9):2029-2036, doi.org/10.1016/j.bpj.2017.08.056
Ignacio Munguira#, Hirohide Takahashi#, Ignacio Casuso & Simon Scheuring*

2017-4 Ignacio Munguira Biophs J-1
2017-4 Ignacio Munguira Biophs J-2

Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis
Nature Cell Biology, 2017, 19(7): Jun 12. doi: 10.1038/ncb3559
Beata Mierzwa#, Nicolas Chiaruttini#, Lorena Redondo-Morata#, Joachim Moser von Filseck, Julia König, Jorge Larios, Ina Poser, Thomas Müller-Reichert, Simon Scheuring, Aurélien Roux* & Daniel Gehrlich*

2017-3 Beata Mierzwa Nature Cell Biology-1
2017-3 Beata Mierzwa Nature Cell Biology-2
2017-3 Beata Mierzwa Nature Cell Biology-3

Dynamin-dependent membrane constriction and fission observed by High-Speed AFM
PNAS, 2017, 114(21):5449-5454. doi: 10.1073/pnas.1619578114
Adai Colom, Lorena Redondo-Morata, Nicolas Chiaruttini, Aurélien Roux* & Simon Scheuring*

2017-2 Adai Colom PNAS-1
2017-2 Adai Colom PNAS-2
2017-2 Adai Colom PNAS-3
2017-2 Adai Colom PNAS-4
2017-2 Adai Colom PNAS-5
2017-2 Adai Colom PNAS-6

Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy
J Mol Biol, 2017, 429(7):977–986; doi.org/10.1016/j.jmb.2017.03.004
Martina Rangl, Luca Rima, Jessica Klement, Atsushi Miyagi, Sandro Keller* & Simon Scheuring*

2017-2 Martina Rangl J Mol Biol-1s

Direct visualization of glutamate transporter elevator mechanism in substrate transport by high-speed AFM
PNAS, 2017, 114(7):1584-1588; doi: 10.1073/pnas.1616413114
Yi Ruan, Atsushi Miyagi, Xiaoyu Wang, Mohamed Chami, Olga Boudker* & Simon Scheuring*

2017-1 Yi Ruan PNAS-1
2017-1 Yi Ruan PNAS-5
2017-1 Yi Ruan PNAS-2
2017-1 Yi Ruan PNAS-6

Temperature-controlled high-speed AFM: Real time observation of ripple-phase transitions.
Small, 2016, 12(44):6106-6113
Hirohide Takahashi#, Atsushi Miyagi# Lorena Redondo-Morata & Simon Scheuring*

2017-6 Hirohide Takahashi Small-4
2017-6 Hirohide Takahashi Small-7
2017-6 Hirohide Takahashi Small-5
2017-6 Hirohide Takahashi Small-1
2017-6 Hirohide Takahashi Small-3
2017-6 Hirohide Takahashi Small-6

Automated force controller for amplitude modulation AFM.
Review of Scientific Instruments, 2016, 87(5): doi.org/10.1063/1.4950777
Atsushi Miyagi* & Simon Scheuring*

2016-5 Atsushi Miyagi Review of Scientific Instruments-1s.gif

Real-time visualization of conformational changes upon ligand unbinding from single MloK1 cyclic nucleotide-modulated channels.
Nature Communications, 2016, 7: doi:10.1038/ncomms12789
Martina Rangl, Atsushi Miyagi, Julia Kowal, Henning Stahlberg, Crina Nimigean* & Simon Scheuring*

2016-4 Martina Rangl Nature Communications-1
2016-4 Martina Rangl Nature Communications-2
2016-4 Martina Rangl Nature Communications-3

High-speed atomic force microscopy shows that annexin-V stabilizes membranes on the second timescale.
Nature Nanotechnology, 2016, 11: 783-790
Atsushi Miyagi, Martina Rangl, Christophe Chipot & Simon Scheuring*

Miyagi,NatureNanotechnology,2016,mov2
Miyagi,NatureNanotechnology,2016,mov3
Miyagi,NatureNanotechnology,2016,mov6
Miyagi,NatureNanotechnology,2016,mov1
Miyagi,NatureNanotechnology,2016,mov5
Miyagi,NatureNanotechnology,2016,mov4
Miyagi,NatureNanotechnology,2016,mov7

Listeriolysin-O membrane damaging activity involves arc formation and lineaction – implication for Listeria monocytogenes escape from phagocytic vacuole.
PLOS Pathogens, 2016, 12(4):e1005597. doi: 10.1371/journal.ppat.1005597
Yi Ruan, Sasa Rezelj, Apolonija Bedina Zavec, Gregor Anderluh* & Simon Scheuring* 

2016-2 Yi Ruan PLOS Pathogens-5
2016-2 Yi Ruan PLOS Pathogens-3
2016-2 Yi Ruan PLOS Pathogens-4

Glass-like membrane protein diffusion in a crowded membrane.
ACS Nano, 2016, 10(2): 2584-2590
Ignacio LB Munguira#, Ignacio Casuso#, Hirohide Takahashi, Felix Rico, Atsushi Miyagi, Mohamed Chami & Simon Scheuring*

2016-1 Ignacio LB Munguira ACS Nano-1
2016-1 Ignacio LB Munguira ACS Nano-4
2016-1 Ignacio LB Munguira ACS Nano-2

Compression of ESCRT-III spiral springs drives membrane deformation.
Cell, 2015, in press
Nicolas Chiaruttini#, Lorena Redondo#, Frédéric Humbert, Martin Lenz*, Simon Scheuring*, Aurélien Roux*

A hybrid high-speed atomic force-optical microscope for visualising single membrane proteins on eukaryotic cells
Nature Communications, 2013, 4: 2155, DOI:10.1038/ncomms3155
Adai Colom#, Ignacio Casuso#, Felix Rico & Simon Scheuring*

2013-1 Adai Colom Nature Communications-2
2013-1 Adai Colom Nature Communications-3
2013-1 Adai Colom Nature Communications-4

High-speed atomic force microscopy: Cooperative adhesion and dynamic equilibrium of junctional microdomain membrane proteins
J Mol Biol, 2012, 423 (2): 249-256
Adai Colom, Ignacio Casuso, Thomas Boudier & Simon Scheuring*

2012-2 Adai Colom J Mol Biol-1
2012-2 Adai Colom J Mol Biol-3
2012-2 Adai Colom J Mol Biol-6

Characterization of the motion of membrane proteins using high speed atomic force microscopy
Nature Nanotechnology, 2012, 7 (8): 525-529
Ignacio Casuso, Jonathan Khao, Mohamed Chami, Perrine Paul-Gilloteaux, Mohamed Husain, Jean-Pierre Duneau, Henning Stahlberg, James N Sturgis & Simon Scheuring* 

2012-1 Ignacio Casuso Nature Nanotechnology-8
2012-1 Ignacio Casuso Nature Nanotechnology-1

Experimental evidence for membrane-mediated protein-protein interaction
Biophys J, 2010, 99 (7): L47-49, doi: 10.1016/j.bpj.2010.07.028
Ignacio Casuso, Pierre Sens, Felix Rico & Simon Scheuring*

2010-1 Ignacio Casuso Biophys J-1
2010-1 Ignacio Casuso Biophys J-2

Contact-mode high-resolution high-speed atomic force microscopy movies of the purple membrane
Biophys J, 2009, 97 (5): 1354-1361
Ignacio Casuso, Noriyuki Kodera, Christian Le Grimellec, Toshio Ando & Simon Scheuring*

2009-1 Ignacio Casuso Biophys J-1
2009-1 Ignacio Casuso Biophys J-2