Key Publications:

A concerted ATPase cycle of the protein transporter AAA-ATPase Bcs1
Nature Communications, 2023, 14, 6369, doi.org/10.1038/s41467-023-41806-5
Yangang Pan, Jingyu Zhan, Yining Jiang, Di Xia, Simon Scheuring*

A pentameric TRPV3 channel with a dilated pore
Nature, 2023, 621(7977), 206–214, doi:10.1038/s41586-023-06470-1
Shifra Lansky, Jingying Zhang, John Michael Betancourt, Yining Jiang, Elizabeth D Kim, Navid Paknejad, Crina M Nimigean, Peng Yuan, Simon Scheuring*

Forces and Energetics of Canonical Tetrameric Cation Channel Gating
PNAS, 2023, 120(28), doi.org/10.1073/pnas.2221616120
Simon Scheuring*

Discrimination between cyclic-nucleotides in a cyclic-nucleotide gated ion channel
Nature Structural & Molecular Biology, 2023, 1545(9985), 10.1038/s41594-023-00955-3
Yangang Pan, Emmi Pohjolainen, Philipp AM Schmidpeter, Andrea C Vaiana, Crina M Nimigean, Helmut Grubmüller, Simon Scheuring*

Membrane-mediated protein interactions drive membrane protein organization
Nature Communications, 2022, 13(7373), doi.org/10.1038/s41467-022-35202-8
Yining Jiang, Batiste Thienpont, Vinay Sapuru, Richard K Hite, Jeremy Dittman, James N Sturgis, Simon Scheuring*

Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism
Nature Communications, 2022, 13(5039). doi.org/10.1038/s41467-022-32757-4
Fang Jiao*, François Dehez, Tao Ni, Xiulian Yu, Jeremy Dittman, Robert Gilbert, Christophe Chipot, Simon Scheuring*

Snf7 spirals sense and alter membrane curvature
Nature Communications, 2022, 13(2174):doi.org/10.1038/s41467-022-29850-z
Nebojsa Jukic#, Alma P. Perrino#, Aurélien Roux & Simon Scheuring*

Single molecule kinetics of bacteriorhodopsin by HS-AFM
Nature Communications, 2021, 12(7225): doi.org/10.1038/s41467-021-27580-2
Alma P. Perrino, Atsushi Miyagi & Simon Scheuring*

Clathrin-coated-pits form from elastically loaded clathrin lattices
Science Advances, 2021, 7(33): doi:10.1126/sciadv.abg9934
Grigory Tagiltsev, Christoph Haselwandter & Simon Scheuring*

Correlation of Membrane Protein Conformational and Functional Dynamics
Nature Communications, 2021, 12(4363), doi.org/10.1038/s41467-021-24660-1
Raghavendar Reddy Sanganna Gari, Joel José Montalvo‐Acosta, George R Heath, Xiaolong Gao, Crina M Nimigean, Christophe Chipot* & Simon Scheuring*

Localization Atomic Force Microscopy
Nature, 2021, 594(7863):385–390, doi:10.1038/s41586-021-03551-x
George Heath, Ekaterina Kots, Janice Robertson, Shifra Lansky, George Khelashvili, Harel Weinstein & Simon Scheuring*

The hierarchical assembly of septins revealed by high-speed AFM
Nature Communications, 2020, 11(5062), doi.org/10.1038/s41467-020-18778-x
Fang Jiao, Kevin S Cannon, Yi-Chih Lin, Amy S Gladfelter & Simon Scheuring*

Millisecond dynamics of an unlabeled amino acid transporter
Nature Communications, 2020, 11(5016), doi.org/10.1038/s41467-020-18811-z
Tina R Matin^#, George R Heath^#, Gerard HM Huysmans, Olga Boudker  & Simon Scheuring*

Annexin-A5 Stabilizes Membrane Defects by Inducing Lipid Phase Transition
Nature Communications, 2020, 11(230), doi:10.1038/s41467-019-14045-w
Yi-Chih Lin, Christophe Chipot & Simon Scheuring*

Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity
Science Advances, 2020, 6(5), doi: 10.1126/sciadv.aax8286
Tao Ni#, Fang Jiao#, Xiulian Yu#, Saša Aden, Lucy Ginger, Sophie Williams, Fangfang Bai, Vojta Prazak, Dimple Karia, Phillip Stansfeld, Peijun Zhang, George Munson, Gregor Anderluh, Simon Scheuring* & Robert JC Gilbert*

Real time dynamics of gating-related conformational changes in CorA
eLife, 2019, 8:e47322 doi: 10.7554/eLife.47322
Martina Rangl, Nicolaus Schmandt, Eduardo Perozo* & Simon Scheuring*

Force-induced conformational changes in Piezo1
Nature, 2019, doi. 10.1038/s41586-019-1499-2
Yi-Chih Lin#, Yusong R Guo#, Atsushi Miyagi, Jesper Levring, Roderick MacKinnon* & Simon Scheuring*

Heterogeneous and rate-dependent streptavidin-biotin unbinding revealed by high-speed force spectroscopy and atomistic simulations
PNAS, 2019, 116(14):6594-6601. doi: 10.1073/pnas.1816909116
Felix Rico#*, Andreas Russek#, Laura Gonzalez, Helmut Grubmüller* & Simon Scheuring*

High-Speed AFM Height Spectroscopy (HS-AFM-HS): Microsecond dynamics of unlabeled biomolecules
Nature Communications, 2018, 9(1):4983. doi:10.1038/s41467-018-07512-3
George Heath & Simon Scheuring*

An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel
Nature Communications, 2018, 9(1):3978. doi:10.1038/s41467-018-06414-8.
Arin Marchesi, Xiaolong Gao, Ricardo Adaixo, Jan Rheinberger, Henning Stahlberg, Crina Nimigean* & Simon Scheuring*

Structural titration of receptor ion channel GLIC gating by HS-AFM
PNAS, 2018, doi.org/10.1073/pnas.1805621115
Yi Ruan, Kevin Kao, Solène Lefebvre, Arin Marchesi, Pierre-Jean Corringer Richard K Hite & Simon Scheuring*

Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis
Nature Cell Biology, 2017, 19(7): Jun 12. doi: 10.1038/ncb3559
Beata Mierzwa#, Nicolas Chiaruttini#, Lorena Redondo-Morata#, Joachim Moser von Filseck, Julia König, Jorge Larios, Ina Poser, Thomas Müller-Reichert, Simon Scheuring, Aurélien Roux* & Daniel Gehrlich*

Dynamin-dependent membrane constriction and fission observed by High-Speed AFM
PNAS, 2017, 114(21):5449-5454. doi: 10.1073/pnas.1619578114
Adai Colom, Lorena Redondo-Morata, Nicolas Chiaruttini, Aurélien Roux* & Simon Scheuring*

High-frequency microrheology reveals cytoskeleton dynamics in living cells
Nature Physics, 2017, doi: 10.1038/nphys4104
Annafrancesca Rigato, Atsushi Miyagi & Simon Scheuring & Felix Rico*

Direct visualization of glutamate transporter elevator mechanism in substrate transport by high-speed AFM.
PNAS, 2017, 114 (7):1584-1588. doi: 10.1073/pnas.1616413114.
Yi Ruan, Atsushi Miyagi, Xiaoyu Wang, Mohamed Chami, Olga Boudker* & Simon Scheuring*

Real-time visualization of conformational changes within single MloK1 cyclic nucleotide-modulated channels.
Nature Communications, 2016, 7: doi:10.1038/ncomms12789
Martina Rangl, Atsushi Miyagi, Julia Kowal, Henning Stahlberg, Crina M Nimigean* & Simon Scheuring*

High-speed atomic force microscopy shows that annexin-V stabilizes membranes on the second timescale.
Nature Nanotechnology, 2016, 11: 783-790
Atsushi Miyagi, Martina Rangl, Chris Chipot & Simon Scheuring*

Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation.
Cell, 2015, 163 (4): 866-879
Nicolas Chiaruttini^#, Lorena Redondo^#, Adai Colom, Frédéric Humbert, Martin Lenz, Simon Scheuring*, Aurélien Roux*

Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1
Nature Communications, 2014, 5: 3106, DOI:10.1038/ncomms4106
Julia Kowal, Mohamed Chami, Paul Baumgartner, Marcel Arheit, Po-Lin Chiu, Martina Rangl, Simon Scheuring, Gunnar F Schröder, Crina M Nimigean*, & Henning Stahlberg*

High-speed force spectroscopy unfolds titin at the velocity of molecular dynamics simulations
Science, 2013, 342 (6159): 741-743
Felix Rico, Laura Gonzalez, Ignacio Casuso, Manel Puig & Simon Scheuring*

Cellular capsules as a tool for multicellular spheroid production and for investigating the mechanics of tumor progression in vitro
PNAS, 2013, 110 (37): 14843-14848
Kévin Alessandri, Bibhu Ranjan Sarangi,Vasily Valérïévitch Gurchenkov, Bidisha Sinha, Tobias Reinhold Kießling, Luc Fetler, Felix Rico, Simon Scheuring, Christophe Lamaze, Anthony Simon, Sara Geraldo, Danijela Vignjević, Hugo Doméjean, Leslie Rolland, Anette Funfak, Jerome Bibette, Nicolas Bremond, Pierre Nassoy

A hybrid high-speed atomic force-optical microscope for visualising single membrane proteins on eukaryotic cells
Nature Communications, 2013, 4: 2155, DOI:10.1038/ncomms3155
Adai Colom, Ignacio Casuso, Felix Rico & Simon Scheuring*

Characterization of the motion of membrane proteins using high speed atomic force microscopy
Nature Nanotechnology, 2012, 7 (8): 525-529
Ignacio Casuso, Jonathan Khao, Mohamed Chami, Perrine Paul-Gilloteaux, Mohamed Husain, Jean-Pierre Duneau, Henning Stahlberg, James N Sturgis & Simon Scheuring*

Mechanical mapping of single proteins at the submolecular level
Nano Letters, 2011, 11 (9): 3983-3986
Felix Rico, Chanmin Su & Simon Scheuring*

Forces guiding assembly of LH2 complexes in native membranes
PNAS, 2011, 108 (23): 9455-9459
Lu-Ning Liu, Katia Duquesne, Filipp Oesterhelt, James N Sturgis & Simon Scheuring*

Antenna mixing in photosynthetic membranes from Phaeospirillum molischianum
PNAS, 2010, 107 (12): 5357-5362
Camille Mascle-Allemand, Katia Duquesne, Regine Lebrun, Simon Scheuring & James N. Sturgis*

The supramolecular architecture of junctional microdomains in native lens membranes
EMBO R, 2007, 8 (1): 51-55
Nikolay Buzhynskyy, Richard K Hite, Thomas Walz, & Simon Scheuring*

2-Chamber-AFM: Probing membrane proteins separating two aqueous compartments
Nature Methods, 2006, 3 (12): 1007-1012
Rui Pedro Gonçalves, Guillaume Agnus, Pierre Sens, Christine Houssin, Bernard Bartenlian, & Simon Scheuring*

Chromatic adaptation of photosynthetic membranes
Science, 2005, 309 (5733): 484-487
Simon Scheuring*, & James Sturgis

Variable LH2 stoichiometry and core clustering in native membranes of Rhodospirillum photometricum
EMBO J, 2004, 23 (21): 4127-4133
Simon Scheuring*, Jean-Louis Rigaud, & James Sturgis

Watching the photosynthetic apparatus in native membranes
PNAS, 2004, 101 (31): 11293-11297
Simon Scheuring*, James N Sturgis, Valerie Prima, Alain Bernadac, Daniel Lévy & Jean-Louis Rigaud

Nanodissection and high-resolution imaging of the photosynthetic core-complex in native membranes of Rps viridis by AFM
PNAS, 2003, 100 (4): 1690-1693
Simon Scheuring*, Jerome Seguin, Sergio Marco, Daniel Lévy, Bruno Robert, & Jean-Louis Rigaud

High resolution AFM topographs of the Rubrivivax gelatinosus light-harvesting complex LH2
EMBO J, 2001, 20 (12): 3029-3035
Simon Scheuring, Francoise Reiss-Husson, Andreas Engel*, Jean-Louis Rigaud & Jean-Luc Ranck

High resolution AFM topographs of the Escherichia coli water channel aquaporin Z
EMBO J, 1999, 18 (18): 4981-4987
Simon Scheuring, Philippe Ringler, Mario Borgnia, Henning Stahlberg, Daniel J Müller, Peter Agre, & Andreas Engel*